Raspberry (Rubus idaeus L.) is a nutrient-rich fruit crop containing high yields of natural bioactive compounds, such as flavonoids and carotenoids, which are known to have potential health benefits in humans. Various colored raspberry fruits offer a unique possibility to study the genetics of pigment biosynthesis in this important soft fruit. Anthocyanidin synthase (Ans) catalyzes the conversion of colorless leucoanthocyanidins to colored anthocyanidins, a key step in biosynthesis of anthocyanins.